sub cloning psnapf Search Results


snap tag  (Addgene inc)
93
Addgene inc snap tag
Snap Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sub+cloning+psnapf/bio_rxiv__2020__04__21__053124-255-8-16?v=Addgene+inc
Average 93 stars, based on 1 article reviews
snap tag - by Bioz Stars, 2026-07
93/100 stars
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psnapf  (New England Biolabs)
95
New England Biolabs psnapf
Psnapf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sub+cloning+psnapf/pmc05522764-646-13-15?v=New+England+Biolabs
Average 95 stars, based on 1 article reviews
psnapf - by Bioz Stars, 2026-07
95/100 stars
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psnap plasmid  (Cisbio Bioassays)
90
Cisbio Bioassays psnap plasmid
Psnap Plasmid, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sub+cloning+psnapf/pmc06737292-539-7-9?v=Cisbio+Bioassays
Average 90 stars, based on 1 article reviews
psnap plasmid - by Bioz Stars, 2026-07
90/100 stars
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sub cloning psnapf  (New England Biolabs)
95
New England Biolabs sub cloning psnapf
Sub Cloning Psnapf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sub+cloning+psnapf/10__7554_slash_elife__92755-412-40-43?v=New+England+Biolabs
Average 95 stars, based on 1 article reviews
sub cloning psnapf - by Bioz Stars, 2026-07
95/100 stars
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e coli expression vector psnap tag t7  (New England Biolabs)
99
New England Biolabs e coli expression vector psnap tag t7
(a) Immunoblot analysis and coomassie blue staining of whole cell extracts made from <t>E.</t> <t>coli</t> cells carrying feline PDE5 expression plasmid pT7-PDE5 or a control vector <t>pSNAP-tag(T7).</t> Lanes 1 and 3 are extracts from cells carrying pSNAP-tag(T7). Lanes 2 and 4 are extracts from cells carrying pT7-PDE5. Lanes 1 and 2 are Coomassie blue staining of the gel. Lanes 3 and 4 are immunoblot using an anti-human PDE5 antibody. (b) Measurement of PDE5 activity. PDE activity of the extracts from cells carrying pT7-PDE5 in the presence or absence of a PDE5 inhibitor, MY5445, was shown (see Method for details). Basal level PDE activity determined with extract from cells carrying pSNAP-tag(T7) was subtracted. The error bars indicate standard deviation (n = 4).
E Coli Expression Vector Psnap Tag T7, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sub+cloning+psnapf/pmc03098252-152-14-19?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
e coli expression vector psnap tag t7 - by Bioz Stars, 2026-07
99/100 stars
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Image Search Results


(a) Immunoblot analysis and coomassie blue staining of whole cell extracts made from E. coli cells carrying feline PDE5 expression plasmid pT7-PDE5 or a control vector pSNAP-tag(T7). Lanes 1 and 3 are extracts from cells carrying pSNAP-tag(T7). Lanes 2 and 4 are extracts from cells carrying pT7-PDE5. Lanes 1 and 2 are Coomassie blue staining of the gel. Lanes 3 and 4 are immunoblot using an anti-human PDE5 antibody. (b) Measurement of PDE5 activity. PDE activity of the extracts from cells carrying pT7-PDE5 in the presence or absence of a PDE5 inhibitor, MY5445, was shown (see Method for details). Basal level PDE activity determined with extract from cells carrying pSNAP-tag(T7) was subtracted. The error bars indicate standard deviation (n = 4).

Journal: PLoS ONE

Article Title: Differential Regulation of PDE5 Expression in Left and Right Ventricles of Feline Hypertrophy Models

doi: 10.1371/journal.pone.0019922

Figure Lengend Snippet: (a) Immunoblot analysis and coomassie blue staining of whole cell extracts made from E. coli cells carrying feline PDE5 expression plasmid pT7-PDE5 or a control vector pSNAP-tag(T7). Lanes 1 and 3 are extracts from cells carrying pSNAP-tag(T7). Lanes 2 and 4 are extracts from cells carrying pT7-PDE5. Lanes 1 and 2 are Coomassie blue staining of the gel. Lanes 3 and 4 are immunoblot using an anti-human PDE5 antibody. (b) Measurement of PDE5 activity. PDE activity of the extracts from cells carrying pT7-PDE5 in the presence or absence of a PDE5 inhibitor, MY5445, was shown (see Method for details). Basal level PDE activity determined with extract from cells carrying pSNAP-tag(T7) was subtracted. The error bars indicate standard deviation (n = 4).

Article Snippet: A NdeI/EcoRI fragment of a mutation-free clone was then excised for sub-cloning into an E. coli expression vector pSNAP-tag(T7) (New England Biolabs, Ipswich, MA) at NdeI and EcoRI site.

Techniques: Western Blot, Staining, Expressing, Plasmid Preparation, Activity Assay, Standard Deviation

Tissue homogenates from LV and RV of control, PA-banded and Ao-banded animal models were analyzed for PDE5 expression by immunoblotting. (a) Homogenates from LV samples. (b) Homogenate from RV samples. Samples from control, PA-banded and Ao-banded groups were indicated in the figure. E. coli extracts from strains carrying pT7-PDE5 and pSNAP-tag(T7) were used as positive and negative controls (shown as+and -). GAPDH levels in the same set of tissue samples were also determined by immunoblotting, and used for normalization of PDE5 levels. (c) Normalized average PDE level in LV tissues from each group (n = 4). (d) Normalized average PDE level in RV tissues from each group. The error bars indicate standard deviations. Student t-test p values that show statistical significance are indicated in the figure.

Journal: PLoS ONE

Article Title: Differential Regulation of PDE5 Expression in Left and Right Ventricles of Feline Hypertrophy Models

doi: 10.1371/journal.pone.0019922

Figure Lengend Snippet: Tissue homogenates from LV and RV of control, PA-banded and Ao-banded animal models were analyzed for PDE5 expression by immunoblotting. (a) Homogenates from LV samples. (b) Homogenate from RV samples. Samples from control, PA-banded and Ao-banded groups were indicated in the figure. E. coli extracts from strains carrying pT7-PDE5 and pSNAP-tag(T7) were used as positive and negative controls (shown as+and -). GAPDH levels in the same set of tissue samples were also determined by immunoblotting, and used for normalization of PDE5 levels. (c) Normalized average PDE level in LV tissues from each group (n = 4). (d) Normalized average PDE level in RV tissues from each group. The error bars indicate standard deviations. Student t-test p values that show statistical significance are indicated in the figure.

Article Snippet: A NdeI/EcoRI fragment of a mutation-free clone was then excised for sub-cloning into an E. coli expression vector pSNAP-tag(T7) (New England Biolabs, Ipswich, MA) at NdeI and EcoRI site.

Techniques: Expressing, Western Blot